Wisconsin Discovery Portal

Researcher: Jean-Michel Ane

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Areas of Research Expertise
  • Understanding and engineering beneficial associations between plants and microbes to improve agricultural productivity, protect the environment over the long term, and enhance human welfare
  • The signals and signaling pathways controlling plant - microbe symbioses
  • Mutualistic associations of plants with arbuscular mycorrhizal (AM) fungi that improve the plants ability to acquire nutrients, especially phosphate and nitrogen, from the soil and their resistance to biotic and abiotic stresses
  • Bacterial signals named Nod factors which are necessary for nodule development and infection of plant roots
  • Plant mutants affected in nodule development, infection thread formation, and early Nod factor responses in the model legume Medicago truncatula
  • DMI1's localization to the nuclear envelope
  • Molecular determinants which explain genetic differences observed between Medicago and Lotus
  • A 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR1) interacting specifically with DMI2/NORK and required for root nodule formation
  • DMI1's control of the colonization of plants by human enteric bacterial pathogens such as Escherichia coli O157:H7 and Salmonella
  • Techniques to characterize the metabolome, proteome and phosphoproteome of M. truncatula
  • A high-throughput procedure based on real-time PCR to quantify soybean rhizobia in the soil in just a few days
  • Impact of drought on the survival of soybean rhizobia in the soil
  • Characterization of Nod and Myc factor signaling in seeds, the characterization of physiological and environmental factors affecting this growth stimulation, and the direct manipulation of symbiotic genes to stimulate plant growth
  • Characterizing rhizobia using their Nod factors to activate the mycorrhizal pathway in cereals (maize, rice…) and colonize them efficiently
  • Improving nitrogen fixation of rhizobia by hosting them in nodule-like structures
Web Site Jean-Michel Ane's Lab Web Page
Curriculum Vitae (CV) Jean-Michel Ane's CV
Current/Active Funding
  • NSF, 2012-2015, An interdisciplinary approach to deciphering the molecular dialogue between the plasma membrane and cytoplasm Of Medicago truncatula
  • NSF, 2013-2016, Collaborative research: understanding and utilizing a unique association between rhizobia and rice
  • Hatch Act Formula Fund, 2013-2017, Poplar as a model to dissect endo- and ecto-mycorrhizal symbioses in wood perennials
  • Hatch Act Formula Fund, 2013-2017, Colonization of legume sprouts by Salmonella enterica
  • Bill and Melinda Gates Foundation, 2012-2017, Engineering the Sym pathway of cereals for recognition of nitrogen fixing bacteria
  • NSF, 2013-2015, Engineering synthetic symbiosis between plant and bacteria to deliver nitrogen to crops
Issued Patent(s)
  • 7,205,450 - DMI1 gene encodes a protein that is required for the early steps of bacterial and fungal symbioses, issued April 2007.
USPTO Published Applications
Recent Publication(s)
  • Comparative phylogenomics uncovers the impact of symbiotic associations on host genome evolution. Delaux P, Varala K, Edger P, Coruzzi G, Pires J, Ané J. PLoS Genet. 2014 July 17;10(7):e1004487. doi: 10.1371/journal.pgen.1004487. eCollection 2014.
  • Plant responses to bacterial N-Acyl l-homoserine lactones are dependent on enzymatic degradation to l-homoserine. Palmer A, Senechal A, Mukherjee A, Ané J, Blackwell H. ACS Chem Biol. 2014 August 15;9(8):1834-1845. doi: 10.1021/cb500191a. Epub 2014 June 18.
  • Staying in touch: mechanical signals in plant-microbe interactions. Jayaraman D, Gilroy S, Ané J. Curr Opin Plant Biol. 2014 August;20C:104-109. doi: 10.1016/j.pbi.2014.05.003. Epub 2014 May 27.
  • Response of Medicago truncatula seedlings to colonization by Salmonella enterica and Escherichia coli O157:H7. Jayaraman D, Valdés-López O, Kaspar C, Ané J. PLoS One. 2014 February 14;9(2):e87970. doi: 10.1371/journal.pone.0087970. eCollection 2014.
Recent Artistic Works
Collaboration
  • Intra-University Collaboration: Chemistry, Botany
  • New York University, Genomics and Systems Biology
  • University of Missouri-Columbia, Bond Life Sciences Center
  • University of California-Berkeley, Plant and Microbial Biology
Research Tools
Research Facilities
E-mail Address [email protected]
Phone Number 608-262-6457
Current University UW–Madison
Department Agronomy
Title Professor
Other Appointments
Address Line 1 348 Moore Hall
Address Line 2 1575 Linden Drive
City Madison
State WI
Zip Code 53706
Bachelor's Degree B.S., University of Lyon, Molecular and Cellular Biology, 1995
Master's Degree M.S., University of Toulouse, Plant Cellular and Molecular Biology, 1997
PhD Ph.D., University of Toulouse, Plant Cellular and Molecular Biology, 2002
Other Degrees
Technologies Available for Licensing