|Areas of Research Expertise||
* Chemical biology, protein design and engineering, enzymology
* Atomic detail of chemical basis and biological purpose for protein structure and function
* Explanantion of biological phenomena by the principles of biochemistry
* Nucleases, including ribonuclease A, Onconase®, and angiogenin
* Protein disulfide isomerase (PDI) and thioredoxin
|Web Site||Ronald Raines' University Web Page|
|Curriculum Vitae (CV)|
|USPTO Published Applications||
Cuchillo CM, Nogues MV, Raines RT, Biochemistry 50(37): 7835-7841 Sep 2011. Here, we celebrate this historic accomplishment by surveying knowledge of enzymology and protein science at that time, facts that led to the formulation of the mechanism, criticisms and alternative mechanisms, data that supported the proposed mechanism, and some of the refinements that have since provided a more precise picture of catalysis of RNA cleavage by ribonucleases.
Choudhary A, Raines RT, Chembiochem 12(12): 1801-1807 Aug 2011. In this Minireview, the electronic, structural, and conformational attributes of four such isosteres-thioamides, esters, alkenes, and fluoroalkenes-are examined in detail.
Smith BD, Higgin JJ, Raines RT, Bioorganic & Medicinal Chemistry Letters 21(17): 5029-5032 Sep 2011. Here, the random acylation of amino groups in wild-type RNase A with folic acid is shown to decrease its catalytic activity dramatically, presumably because of the alteration to a key active-site residue, Lys41.
Rutkoski TJ, Kink JA, Strong LE, et al., Cancer Biology & Therapy 12(3): 208-214 Aug 2011. We reasoned that site-specific conjugation of a poly(ethylene glycol) (PEG) chain could both reduce sensitivity to RI and increase serum half-life.
|Recent Artistic Works|
|E-mail Address||[email protected]|
|Phone Number||(608) 262-8588|
|Current University||UW - Madison|
|Department||Biochemistry / Chemistry|
Director, Biophysics Instrumentation Facility
|Address Line 1||371B Biochemistry Addition|
|Address Line 2||433 Babcock Drive|
|Bachelor's Degree||ScB, Massachusetts Institute of Technology, Biology and Chemistry, 1980|
|Master's Degree||AM, Harvard University, Chemistry, 1982|
|PhD||PhD, Harvard University, Chemistry, 1986|
|Technologies Available for Licensing||
Strong, Stable, Semisynthetic Collagen Mimic for Wound Healing, Artificial Skin, Sutures and Leather