• DHHS, PHS, NIH, 2009-2012, Microchannel Cell-Based Assays to Enable Cancer Research
• DOD, Army, 2009-2012, Arrayed Microchannel-Based Assays for Circulating Turmor Cell Capture, Culture, and Analysis
• NIH, 2007-2010, Microfluidic Channels for High Density, High Performance Culture Assays
• Wisconsin Alumni Research Foundation, 2006-2033
• University of California, DOD, 2006-2009, Microfluidics and Cellular Assays
• Wisconsin Alumni Research Foundation, 2005-2032
• NIH, 2004-2009, Study of Progenitor Regulation in Mouse Mammary Gland
• Wisconsin Alumni Research Foundation, 2004-2010, Romnes Faculty Fellow
• American Medical Systems, Inc., 2003-2030, Novel Approaches to Drug Delivery

• 7,504,069 - Micro device for high resolution delivery and monitoring of stimuli to a biological object, in vitro, granted Mar 2009.
• 7,470,403 - Microfluidic platform and method of generating a gradient, granted Dec 2008.
• 7,277,021 - Device and method for alerting a runner when a new pair of running shoes is needed, granted Oct 2007.
• 7,253,003 - Method for monitoring the environment within a microfluidic device, granted Aug 2007.
• 7,189,580 - Method of pumping fluid through a microfluidic device, granted Mar 2007.

• "Fundamentals of microfluidic cell culture in controlled microenviornments"

Young EWK, Beebe DJ, Chemical Society Reviews 39 (3): 1036-1048 2010. This tutorial review integrates fundamental principles from cell biology and local microenvironments with cell culture techniques and concepts in microfluidics. Culturing cells in microscale environments requires knowledge of multiple disciplines including physics, biochemistry, and engineering. We discuss basic concepts related to the physical and biochemical microenvironments of the cell, physicochemical properties of that microenvironment, cell culture techniques, and practical knowledge of microfluidic device design and operation. We also discuss the most recent advances in microfluidic cell culture and their implications on the future of the field.

• "Control of 3-dimensional collagen matrix polymerization for reproducible human mammary fibroblast cell culture in microfluidic devices"

SUng KE, Su G, Beebe DJ et al. Biomaterials 30(27): 5833-4841 September 2009. Interest in constructing a reliable 3-dimensional (3D) collagen culture platform in microfabricated systems is increasing as researchers strive to investigate reciprocal interaction between extracellular matrix (ECM) and cells under various conditions. However, in comparison to conventional 2-dimensional (2D) cell culture research, relatively little work has been reported about the polymerization of collagen type I matrix in microsystems. We, thus, present a study of 3D collagen polymerization to achieve reproducible 3D cell culture in microfluiclic devices.

• "Biological implications of polydimethylsiloxane-based microfluidic cell culture"

Beebe DJ, Murphy WL, Alarid ET et al. Lab on a Chip 9(15): 2132-2139 2009. Polydimethylsiloxane (PDMS) has become a staple of the microfluidics community by virtue of its simple fabrication process and material attributes, such as gas permeability, optical transparency, and flexibility.

• "Biological implications of polydimethylsiloxane-based microfluidic cell culture"

Regehr KJ, Domenech M, Koepsel JT, et al., Lab Chip 9 (15): 2132-2139 Aug 2009. Two properties of PDMS were addressed in this study: the leaching of uncured oligomers from the polymer network into microchannel media, and the absorption of small, hydrophobic molecules (i.e. estrogen) from serum-containing media into the polymer bulk. Pubmed/NLM.

• Intra-University Collaboration: Electrical and Computer Engineering, Pediatrics, Pharmacology, Animal Sciences, Orthopedics and Rehabilitation Medicine, Mechanical Engineering, Biochemistry, Biology & Chemical Engineering
• Vitae LLC, Kathyrn Haubert
• Phillips Medical Systems, Krishnakant Nammi
• University of Illinios at Urbana-Champaign, Veterinary Clinical Medicine
• Northwestern University, Biomedical Engineering
• Dankook University (Korea), Biomedical Engineering
• Shippensburg University, Psychology

• BrightLine DA/FI/TX-3X-A Triple-Band "Pinkel" Filter Set
• Acticure
• Spectrophotometer
• Goniometer

• Microtechnology Medicine Biology Lab
• UW Carbone Cancer Center
• Biomedical Engineering Center for Translational Research