- "RAB-11 permissively regulates spindle alignment by modulating metaphase microtubule dynamics in Caenorhabditis elegans early embryos"
Zhang H, Squirrell JM, White JG, Mol Biol Cell 19 (6): 2553-2565 Jun 2008. We hypothesize that RAB-11, in conjunction with the ER, acts to regulate cell cycle-specific changes in astral microtubule length to ensure proper spindle alignment in Caenorhabditis elegans early embryos. Pubmed.
- "Collagen density promotes mammary tumor initiation and progression"
Provenzano PP, Inman DR, Eliceiri KW, et al., BMC Medicine 6 (11): Apr 2008. To study the effects of collagen density on mammary tumor formation and progression, we utilized a bi-transgenic tumor model with increased stromal collagen in mouse mammary tissue. Imaging of the tumors and tumor-stromal interface in live tumor tissue was performed with multiphoton laser-scanning microscopy to generate multiphoton excitation and spectrally resolved fluorescent lifetimes of endogenous fluorophores. Second harmonic generation was utilized to image stromal collagen.
- "Src and Wnt signaling regulate dynactin accumulation to the P2-EMS cell border in C. elegans embryos"
Zhang H, Skop AR, White JG, Journal of Cell Science 121 (2): 155-161 Jan 2008. Here, we describe how components of the dynactin complex became locally enriched at the P2-EMS border prior to and during rotational alignment of their spindles. Wnt and Src signaling were required for both localized dynactin enrichment, and for rotational alignment of the P2 and EMS spindles.
- "In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia"
Skala MC, Riching KM, Gendron-Fitzpatrick A, et al., Proceedings of the National Academy of Sciences of the United States of America 104 (49): 19494-19499 Dec 2007. This study combines cellular redox ratio, NADH and FAD lifetime, and subcellular morphology imaging in three dimensions to identify intrinsic sources of metabolic and structural contrast in vivo at the earliest stages of cancer development. There was a significant (P < 0.05) increase in the nuclear to cytoplasmic ratio (NCR) with depth within the epithelium in normal tissues; however, there was no significant change in NCR with depth in precancerous tissues.