- "Biochemical and functional analysis of TIR domain containing protein from Brucella melitensis"
Radhakrishnan GK, Splitter GA, Biochecmical and Biophysical Research Communications 397 (1): 59-63 Jun 2010. Toll/interleukin-1 like receptors are evolutionarily conserved proteins in eukaryotes that play crucial role in pathogen recognition and innate immune responses. With the objective of detailed characterization, we have over expressed and purified TcpB from Brucella melitensis in native condition. The purified protein exhibited lipid-binding properties and cell permeability.
- "Discordant Brucella melitensis antigens yield cognate CD8(+) T Cells in vivo"
Durward MA, Harms J, Magnani DM, Eskra L, Splitter GA, Infection and Immunity 78(1): Jan 2010. The authors found by microarray analysis that the genes encoding these epitopes are differentially expressed following macrophage infection, further emphasizing that these discordant genes may play an important role in the pathogenesis of B. melitensis infection.
- "Brucella regulators: self-control in a hostile environment"
Rambow-Larsen AA, Petersen EM, Splitter GA et al. Trends in Microbiology 17(8): 371-377 August 2009. Brucella is an important zoonotic pathogen for which no human vaccine exists. In an infected host, Brucella resides in macrophages but must coordinate expression of multiple virulence factors for successful cell entry and trafficking to acquire this replicative niche.
- "DNA recognition properties of the cell-to-cell movement protein (MP) of soybean isolate of Mungbean yellow mosaic India virus (MYMIV-Sb)"
Radhakrishnan GK, Splitter GA, Usha R, Virus Research 131 (2): 152-159 Feb 2008. To investigate the nucleic acid recognition properties of MP of MYMIV-Sb, the protein was expressed in Escherichia coli as a fusion protein with maltose-binding protein (MBP) and purified in native condition. Gel mobility shift assay was employed for analyzing the DNA recognition properties of purified MBP-MP fusion protein.